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AbstractTo investigate structure and biological properties of the nucleocapsid (N) protein of respiratory syncytial virus (RSV), we have generated a panel of 16 monoclonal antibodies steroids, raised against recombinant N protein steroids, and epitope mapped seven of these to three antigenic sites (Site I aa 16 30; Site II aa 341 350; Site III aa 351 365). Characterization by immunofluorescence and by immunoprecipitation assay demonstrated that a monoclonal antibody to antigenic site I can detect N protein complexed with phospho (P) protein. Antibodies to antigenic sites II and III steroids, which are adjacent to each other near the carboxyl terminus of the N protein, have distinct properties.
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